Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Kit
Cov khoom siv PCR zoo heev, npaj rau siv no muaj nyob rau hauv hom lyophilized (txheej txheej txheem kom qhuav) rau lub sijhawm ntev.Cov khoom siv tuaj yeem thauj thiab khaws cia hauv chav sov thiab ruaj khov rau ib xyoos.Txhua lub raj ntawm premix muaj tag nrho cov reagents xav tau rau PCR amplification, suav nrog Reverse-transcriptase, Taq polymerase, primers, probes, thiab dNTPs substrates.Nws tsuas yog xav tau ntxiv 13ul distilled dej thiab 5ul rho tawm RNA template, ces nws tuaj yeem khiav thiab nthuav dav ntawm PCR cov cuab yeej.
Lub tshuab qPCR yuav tsum tau ntsibcov cai hauv qab no:
1. Haum 8 strip PCR raj ntim 0.2 ml
2. Muaj ntau tshaj plaub qhov kev kuaj pom:
| Channel | Excitation (nm) | Emission (nm) | Pre-Calibrated Dyes |
| 1. | 470 | 525 | FAM, SYBR Green I |
| 2 | 523 ib | 564 ib | VIC, HEX, TET, JOE |
| 3. | 571 ib | 621 ib | ROX, TEXAS-RED |
| 4 | 630 | 670 ib | CY 5 |
PCR-platforms:
7500Real-Time PCR System, Biorad CF96, iCycler iQ™ Real-Time PCR Detection System, Stratagene Mx3000P, Mx3005P
Qhov nyuaj ntawm kev thauj mus los txias ntawmNovel Coronavirus nucleic acid detection reagent
Thaum cov pa nucleic acid kuaj pom cov reagents raug thauj mus rau qhov ntev, qhov (-20 ± 5) ℃ txias saw thiab tsheb thauj mus los yuav tsum tau ua kom ntseeg tau tias bioactive enzyme hauv cov reagents tseem ua haujlwm.Txhawm rau kom paub tseeb tias qhov ntsuas kub nce mus txog tus qauv, ob peb kilograms ntawm cov dej khov qhuav yog xav tau rau txhua lub thawv ntawm nucleic acid kuaj reagent txawm tias tsawg dua 50g, tab sis nws tuaj yeem kav ntev li ob lossis peb hnub.Ntawm qhov kev xav ntawm kev lag luam kev xyaum, qhov tseeb qhov hnyav ntawm cov tshuaj reagents tawm los ntawm cov tuam txhab lag luam yog tsawg dua 10% (lossis deb tshaj qhov nqi no) ntawm lub thawv.Feem ntau ntawm qhov hnyav yog los ntawm cov dej khov qhuav, cov pob khov nab kuab thiab cov thawv ua npuas dej, yog li tus nqi tsheb thauj mus los yog siab heev.
Thaum Lub Peb Hlis 2020, COVID-19 tau pib tawg tawm hauv qhov loj nyob txawv teb chaws, thiab qhov kev thov rau Novel Coronavirus nucleic acid nrhiav pom cov tshuaj reagent tau nce ntau.Txawm hais tias tus nqi siab ntawm kev xa tawm cov reagents nyob rau hauv cov kab mob khaub thuas, feem ntau cov tuam ntxhab tseem tuaj yeem txais nws vim qhov ntau thiab cov nyiaj tau los.
Txawm li cas los xij, nrog kev txhim kho cov cai xa tawm hauv tebchaws rau cov khoom lag luam tiv thaiv kev sib kis, nrog rau kev txhim kho kev tswj hwm lub teb chaws ntawm kev khiav ntawm tib neeg thiab kev thauj mus los, muaj qhov txuas ntxiv thiab tsis paub tseeb hauv lub sijhawm thauj mus los ntawm cov tshuaj reagents, uas ua rau cov khoom lag luam muaj teeb meem tshwm sim. los ntawm kev thauj mus los.Kev ncua sij hawm thauj mus los (lub sijhawm thauj mus los txog li ib nrab ib hlis yog qhov ntau heev) ua rau cov khoom lag luam tsis tu ncua thaum cov khoom mus txog tus neeg siv khoom.Qhov no tau teeb meem feem ntau nucleic acid reagents export lag luam.
Lyophilized thev naus laus zis rau PCR reagent pab kev thauj mus los ntawmNovel Coronavirus nucleic acid kuaj pom reagent thoob ntiaj teb
Cov lyophilized PCR reagents tuaj yeem thauj thiab khaws cia hauv chav tsev kub, uas tuaj yeem tsis tsuas yog txo tus nqi thauj mus los, tab sis kuj zam cov teeb meem zoo los ntawm kev thauj mus los.Yog li ntawd, lyophilizing cov reagent yog qhov zoo tshaj plaws los daws qhov teeb meem ntawm kev thauj mus los.
Lyophilization suav nrog kev tso dej khov rau hauv lub xeev khov, thiab tom qab ntawd sublimate thiab cais cov dej vapor nyob rau hauv lub tshuab nqus tsev.Lub qhuav solute tseem nyob rau hauv lub thawv nrog tib yam muaj pes tsawg leeg thiab kev ua si.Piv nrog rau cov kua reagents ib txwm, cov khoom siv tag nrho lyophilized Novel Coronavirus nucleic acid nrhiav reagent tsim los ntawm Liming Bio muaj cov yam ntxwv hauv qab no:
Muaj zog kub stability:Nws tuaj yeem nrog kev kho mob ntawm 56 ℃ rau 60 hnub, thiab morphology thiab kev ua haujlwm ntawm cov reagent tseem tsis hloov pauv.
Qhov kub thiab txias cia thiab kev thauj mus los:tsis xav tau cov hlua txias, tsis tas yuav khaws cia ntawm qhov kub thiab txias ua ntej unsealing, tag nrho tso tawm qhov chaw txias txias.
Npaj-rau-siv:lyophilizing ntawm tag nrho cov Cheebtsam, tsis tas yuav tsum tau rau lub system configuration, tsis txhob poob ntawm lub Cheebtsam nrog siab viscosity xws li enzyme.
Multiplex hom phiaj nyob rau hauv ib lub raj:Lub hom phiaj tshawb nrhiav npog cov kab mob tshiab coronavirus ORF1ab gene, N gene, S gene kom tsis txhob muaj tus kab mob genovariation.Txhawm rau txo qis qhov tsis zoo, tib neeg RNase P noob tau siv los ua kev tswj hwm sab hauv, txhawm rau ua kom tau raws li qhov xav tau kev kho mob rau cov qauv kev tswj hwm zoo.
